ABSTRACT
Objective To explore the influences of sufentanil and different dose midazolam coprocessing on myocardial injury in rats during myocardial ischemia-reperfusion (IR ). Methods Forty two healthy male SD rats were selected and randomly divided into 7 groups including,group A:rats were treated with sham operation;groups B0-B5:rat IR model was con-structed;group B0:rats were untreated with sufentanil or midazolam;group B1:rats were per-trea-ted with 3 μg/kg sufentanil;group B2:rats were post-treated with 0.1 mg/kg midazolam;group B3:rats were post-treated with 0.3 mg/kg midazolam;group B4:rats were per-treated with 1.5 μg/kg sufentanil and post-treated with 0.05 mg/kg midazolam;group B5:rats were per-treated with 1.5μg/kg sufentanil and post-treated with 0.15 mg/kg midazolam.Sufentanil was injected through cervi-cal vein before ischemia.Midazolam was injected through cervical vein after ischemia.After reperfu-sion,arterial blood was collected for detecting the activity of CK-MB,LDH or SOD,and the concen-tration of MDA,TNF-α,or IL-6.Rat hearts were picked off to measure the severity of myocardial in-farction (IS/AAR).The expressions of Bax,cleaved-caspase-3 and Bcl-2 in myocardial tissues were detected by Western blots.Results CK-MB activity,LDH activity,MDA concentration,TNF-αlev-el,IL-6 level,Bax and cleaved-caspase 3 expression levels in groups B0-B5 were obviously higher than those in group A (P <0.05),in groups B1-B5 were obviously lower than those in group B0 (P<0.05),in groups B3 and B4 were obviously lower than those in group B2 (P <0.05),in group B5 were obviously lower than those in groups B1-B4 (P < 0.05 );values of IS and IS/AAR (%)in groups B1-B5 were obviously lower than those in group B0 (P <0.05),in groups B3 and B4 were ob-viously lower than those in group B2 (P < 0.05 ),in group B5 were obviously lower than those in groups B1-B4 (P <0.05 );SOD activity and Bcl-2 expression level in groups B0-B5 were obviously lower than those in group A (P <0.05),in groups B1-B5 were obviously higher than those in group B0 (P <0.05),in groups B3 and B4 were obviously higher than those in group B2 (P <0.05 ),in group B5 were obviously higher than those in groups B1-B4 (P <0.05).CK-MB declined when com-pared with group B0;LDH declined;SOD rose;MDA declined;TNF-αdeclined;IL-6 declined;IS/AAR declined;Bcl-2 expression rose to 2.25 fold;Bax expression dropped to 54.89%;cleaved-caspase-3 expression dropped to 49.67%.Conclusion Sufentanil pretreatment combined with mid-azolam aftertreatment can significantly alleviate rat myocardial IR injury,which plays more effective cardioprotective effects than being used alone.